Equine abortion associated with placentitis caused by Pasteurella pneumotropica / Aborto equino associado a placentite por Pasteurella pneumotropica

Pasteurella pneumotropica is a bacterium that has so far not been described as a cause of placentitis in animals. Two cases of aborted equine fetuses were sent to the Department of Veterinary Pathology of the "Universidade Federal do Rio Grande do Sul" (SPV-UFRGS) for anatomopathological examination. Both cases presented suppurative placentitis associated with multiple basophilic bacterial cells. After bacterial isolation and biochemical analysis, P. pneumotropica was identified.

Pasteurella pneumotropica é uma bactéria que até o momento não foi descrita como causa de placentite em animais. Dois casos de fetos equinos abortados foram enviados ao Setor de Patologia Veterinária da Universidade Federal do Rio Grande do Sul (SPV-UFRGS) para exame anatomopatológico. Em ambos os casos se observou placentite supurativa associada a múltiplas colônias bacterianas basofílicas. Após o isolamento bacteriano e análise bioquímica, indentificou-se P. pneumotropica.

Equine abortion associated with placentitis caused by Pasteurella pneumotropica / Aborto equino associado a placentite por Pasteurella pneumotropica

Pasteurella pneumotropica is a bacterium that has so far not been described as a cause of placentitis in animals. Two cases of aborted equine fetuses were sent to the Department of Veterinary Pathology of the "Universidade Federal do Rio Grande do Sul" (SPV-UFRGS) for anatomopathological examination. Both cases presented suppurative placentitis associated with multiple basophilic bacterial cells. After bacterial isolation and biochemical analysis, P. pneumotropica was identified.(AU)

Pasteurella pneumotropica é uma bactéria que até o momento não foi descrita como causa de placentite em animais. Dois casos de fetos equinos abortados foram enviados ao Setor de Patologia Veterinária da Universidade Federal do Rio Grande do Sul (SPV-UFRGS) para exame anatomopatológico. Em ambos os casos se observou placentite supurativa associada a múltiplas colônias bacterianas basofílicas. Após o isolamento bacteriano e análise bioquímica, indentificou-se P. pneumotropica.(AU)

Enterobacteriaceae and pseudomonadaceae on the dorsum of the human tongue

OBJECTIVE: The aim of this study was to correlate the presence of Enterobacteriaceae, Pseudomonadaceae, Moraxellaceae and Xanthomonadaceae on the posterior dorsum of the human tongue with the presence of tongue coating, gender, age, smoking habit and denture use. MATERIAL AND METHODS: Bacteria were isolated from the posterior tongue dorsum of 100 individuals in MacConkey agar medium and were identified by the API 20E system (Biolab-Mérieux). RESULTS: 43 percent of the individuals, presented the target microorganisms on the tongue dorsum, with greater prevalence among individuals between 40 and 50 years of age (p = 0.001) and non-smokers (p=0.0485). CONCLUSIONS: A higher prevalence of Enterobacteriaceae and Pseudomonadaceae was observed on the tongue dorsum of the individuals evaluated. There was no correlation between these species and the presence and thickness of tongue coating, gender and presence of dentures.

Pasteurella pneumotropica causa la regresion de tumores humanos trasplantados en ratones inmunodeficientes / Pasteurella pneumotropica produces regression of human tumors transplanted in immunodeficiency mice

La técnica de trasplante de tumores humanos en ratones inmunodeficientes es muy utilizada como modelo en investigaciones sobre el cáncer. De acuerdo con las recomendaciones internacionales, los animales de experimentación deben estar libres de los micoorganismos que interfieren en los resultados finales de las investigaciones, dentro de los cuales se encuentra Pasteurella pneumotropica. En el presente trabajo se evaluó la interferencia que produce esta bacteria en el crecimiento de la línea celular A549 de adenocarcinoma humano trasplantada en ratones de la cepa nude N:NIH (S)-nu. Se utilizaron 40 ratones divididos en 4 grupos de 10 animales cada uno. Grupo 1: inoculados con la línea celular; grupo 2, con la bacteria; grupo 3, con la línea celular y la bacteria y grupo 4, el control sin inoculaciones. Se observaron diferencias significativas en el crecimiento tumoral entre los animales de los grupos 1 y 3. Si bien este microorganismo es un patógeno oportunista no letal, los ratones trasplantados con la línea celular A549 e infectados con P. pneumotropica no son aptos para utilizarse como modelo animal en estudios sobre el cáncer debido a que esta bacteria interfiere en el desarrollo de la línea tumoral, con la consecuente interpretación errónea de los resultados. Pero el hecho que la bacteria haya causado la regresión de un tumor en pleno crecimiento es inesperado y el mecanismo de acción será objeto de futuros experimentos.

The technique of human tumor cell line transplantation in immunodeficient miceis used worldwide as a model for cancer research. In accordance with international recommendations, animals used in biomedical research should be free of microorganisms which can interfere in experimental results; including Pasteurella pneumotropica. The object of this study was to evaluate the interference produced by P. pneumotropica in the human adenocarcinoma cell line A549 transplanted in N:NIH(S)-nu mice. A total of 40 mice divided into 4 groups of 10 animals each was used to perform this study. Group 1: inoculated with the cell line; group 2, with the bacteria; group 3, with the cell line and the bacteria; group 4, as control with no inoculations. Significant differences were observed in tumor growth in groups 1 and 3, infected and not infected with P. pneumotropica. Although this microorganism is non lethal and only opportunistic, the infected animals are to be considered not suitable to be transplanted with the tumor cell line A549 for experimental studies since these bacteria interfere with tumor growth. However, the fact that a growing tumor regresses in the presence of the bacteria is an interesting bservation which deserves further exploration in order to elucidate the mechanism involved.